Crystallization and preliminary X‐ray diffraction analysis of staphylococcal enterotoxin type C

Gregory A. Bohach, Young‐In ‐I Chi, Cynthia V. Stauffacher

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11 Scopus citations

Abstract

The Type C staphylococcal enterotoxin produced by Staphylococcus aureus strain FRI‐909 has been crystallized using a combination of two precipitants, ammonium sulfate and polyethylene glycol 400, with the addition of small amounts of detergent. Two related crystal forms have been obtained, one triclinic, and one tetragonal, both with one toxin molecule per asymmetric unit. These crystals are stable for at least 75 hr in the X‐ray beam and diffract to at least 2.2 and 2.6 Å, respectively. The triclinic crystals have unit cell parameters a = 38.5 Å, b = 43.7 Å, c = 36.9 Å, and interaxial angles α = 99.9°, β = 95.8°, and γ = 98.5°. The tetragonal crystals are of space group P4122 with unit cell parameters a = 43.4 Å and c = 278.0 Å. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)152-157
Number of pages6
JournalProteins: Structure, Function, and Bioinformatics
Volume13
Issue number2
DOIs
StatePublished - Jun 1992

Keywords

  • Staphylococcus aureus
  • bacterial toxins
  • crystallography
  • protein structure
  • pyrogenic toxins
  • structure–function

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