Crystallization and preliminary X-ray data of proteins derived from prothrombin

w. Hu Kung, A. Tulinsky, G. L. Nelsestuen

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Crystals of bovine prothrombin fragment 1 and deglycosylated fragment 1 have been grown from polyethylene glycol, Tris-maleate solutions and the crystals scatter x-rays at 2.8 Å resolution. The crystals of both are tetragonal, space group P41212, or P43212, with 1 molecule per asymmetric unit. Moreover, the two appear to be isomorphous. Since the mother liquor can support Ca2+ ion without precipitation, crystals have been soaked with Ca2+ ion and preliminary results indicate that these remain closely isomorphous with parent crystals. Isomorphous heavy atom derivatives of mercury and platinum have been prepared of glycosylated fragment 1. In addition, crystals of human prothrombin fragment 1 have also been grown.

Original languageEnglish (US)
Pages (from-to)10523-10525
Number of pages3
JournalJournal of Biological Chemistry
Issue number21
StatePublished - 1980


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