Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding

Ryan R. Davisy, Nadine M. Shaban, Fred W. Perrino, Thomas Hollis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

RNA-DNA hybrids play essential roles in a variety of biological processes, including DNA replication, transcription, and viral integration. Ribonucleotides incorporated within DNA are hydrolyzed by RNase H enzymes in a removal process that is necessary for maintaining genomic stability. In order to understand the structural determinants involved in recognition of a hybrid substrate by RNase H we have determined the crystal structure of a dodecameric nonpolypurine/ polypyrimidine tract RNA-DNA duplex. A comparison to the same sequence bound to RNase H, reveals structural changes to the duplex that include widening of the major groove to 12.5 A from 4.2 Å and decreasing the degree of bending along the axis which may play a crucial role in the ribonucleotide recognition and cleavage mechanism within RNase H. This structure allows a direct comparison to be made about the conformational changes induced in RNA-DNA hybrids upon binding to RNase H and may provide insight into how dysfunction in the endonuclease causes disease.

Original languageEnglish (US)
Pages (from-to)668-673
Number of pages6
JournalCell Cycle
Volume14
Issue number4
DOIs
StatePublished - Feb 15 2015

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Ribonuclease H
RNA
DNA
Ribonucleotides
Virus Integration
Biological Phenomena
Genomic Instability
Endonucleases
DNA Replication
Enzymes

Keywords

  • Conformational changes
  • Protein nucleic acid interaction
  • RNA-DNA hybrid
  • RNase H

Cite this

Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding. / Davisy, Ryan R.; Shaban, Nadine M.; Perrino, Fred W.; Hollis, Thomas.

In: Cell Cycle, Vol. 14, No. 4, 15.02.2015, p. 668-673.

Research output: Contribution to journalArticle

Davisy, Ryan R. ; Shaban, Nadine M. ; Perrino, Fred W. ; Hollis, Thomas. / Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding. In: Cell Cycle. 2015 ; Vol. 14, No. 4. pp. 668-673.
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