TY - JOUR
T1 - Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)
AU - Chelladurai, Jeba Jesudoss
AU - Clark, Mark E.
AU - Kváč, Martin
AU - Holubová, Nikola
AU - Khan, Eakalak
AU - Stenger, Brianna L.S.
AU - Giddings, Catherine W.
AU - McEvoy, John
N1 - Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.
AB - Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.
KW - Avian genotype VI
KW - Cryptosporidium
KW - Cryptosporidium galli
KW - Intestine
KW - Passerines
KW - Proventriculus
KW - Red-winged blackbird
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U2 - 10.1007/s00436-016-4930-8
DO - 10.1007/s00436-016-4930-8
M3 - Article
C2 - 26818945
AN - SCOPUS:84955611877
SN - 0932-0113
VL - 115
SP - 1901
EP - 1906
JO - Parasitology Research
JF - Parasitology Research
IS - 5
ER -