Cryptococcal phosphoglucose isomerase is required for virulence factor production, cell wall integrity and stress resistance

Ping Zhang, Dongsheng Wei, Zhongming Li, Zhixiong Sun, Jiao Pan, Xudong Zhu

Research output: Contribution to journalEditorial

9 Scopus citations

Abstract

Regulation of virulence factor production in the pathogen Cryptococcus neoformans remains to be fully illustrated. We present here a finding that a gene, encoding the glycolysis enzyme phosphoglucose isomerase (Pgi1), is critical for the biosynthesis of melanin and capsule, cell wall integrity and resistance to stress conditions. A leaky mutant of the yeast, LZM19, resulted from an insertion of T-DNA in the PGI1 promoter region, expressed PGI1 at a level only 1.9% of the wild type. LZM19 could synthesize the pigment melanin in the presence of 2% glucose, suggesting a status of LAC1 derepression. Phenotypically, capsule biosynthesis in LZM19 was remarkably reduced. Integrity of the cell wall and plasma membrane of LZM19 were impaired based on its sensitivity to Congo red and SDS. Also, LZM19 exhibited hypersensitivity to osmotic stress generated by 2 M NaCl or 1 M KCl, indicating possible impairment in the HOG signaling pathway. Furthermore, LZM19 failed to utilize mannose and fructose, suggesting a possible involvement of Pgi1 in the breakdown of these two sugars. Our results revealed a crucial role of PGI1 in coordination of the production of virulence factors, cell wall integrity and stress response in C. neoformans.

Original languageEnglish (US)
JournalFEMS yeast research
Volume15
Issue number7
DOIs
StatePublished - Nov 1 2015

Keywords

  • Cryptococcus neoformans
  • HOG pathway
  • capsule
  • laccase
  • melanin
  • phosphoglucose isomerase

Fingerprint Dive into the research topics of 'Cryptococcal phosphoglucose isomerase is required for virulence factor production, cell wall integrity and stress resistance'. Together they form a unique fingerprint.

  • Cite this