Cryopreservation of hematopoietic and non-hematopoietic stem cells

K. K. Fleming, Allison Hubel

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Recent studies illustrate the potential for improving the cryopreservation of stem cells. Reduced DMSO concentrations in the cryopreservation medium, post thaw washing of cells and increased cell concentration have been actively studied. Standardization of cell processing has led to the study of liquid storage prior to cryopreservation, validation of mechanical (uncontrolled rate freezing) freezing, and cryopreservation bag failure. Finally, the need for the systematic study and optimization of preservation processes has not been fulfilled. As the sources and applications of stem cells (hematopoietic and non-hematopoietic) continue to be developed, the need for effective preservation methods will only grow.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalTransfusion and Apheresis Science
Volume34
Issue number3
DOIs
StatePublished - Jun 1 2006

Keywords

  • Cell processing
  • Dimethylsulfoxide
  • Stromal cells
  • Trehalose

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