Cryopreservation of cultured blood cells for use in gene therapy and immunotherapy

Allison Hubel, Jeffrey McCullough

Research output: Contribution to journalArticlepeer-review

Abstract

The freezing characteristics of genetically manipulated lymphocytes and hematopoietic stem cells were studied. The water transport characteristics of stem cells which had been cultured and transduced with a therapeutic gene were different from that of a freshly isolated cells. Changes in the freezing characteristics for genetically transformed lymphocytes were also observed. Specifically, cryopreservation protocols developed for the fresh peripheral blood lymphocytes do not produce comparable survival rates for lymphocytes which have been cultured and transduced. These results indicate that there are significant changes in the freezing characteristics resulting from the culture and transduction process.

Original languageEnglish (US)
Pages (from-to)97-98
Number of pages2
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume37
StatePublished - Dec 1 1997

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