Cryogenic "iodide-Tagging" Photoelectron Spectroscopy: A Sensitive Probe for Specific Binding Sites of Amino Acids

Hanhui Zhang, Hanhui Zhang, Wenjin Cao, Qinqin Yuan, Xiaoguo Zhou, Marat Valiev, Steven R. Kass, Xue Bin Wang

Research output: Contribution to journalArticle

Abstract

This work showcases cryogenic and temperature-dependent "iodide-tagging"photoelectron spectroscopy to probe specific binding sites of amino acids using the glycine-iodide complex (Gly·I-) as a case study. Multiple Gly·I- isomers were generated from ambient electrospray ionization and kinetically isolated in a cryogenic ion trap. These structures were characterized with temperature-dependent "iodide-tagging"negative ion photoelectron spectroscopy (NIPES), where iodide was used as the "messenger"to interpret electronic energetics and structural information of various Gly·I- isomers. Accompanied by theoretical computations and Franck-Condon simulations, a total of five cluster structures have been identified along with their various binding motifs. This work demonstrates that "iodide-tagging"NIPES is a powerful general means for probing specific binding interactions in biological molecules of interest.

Original languageEnglish (US)
Pages (from-to)4346-4352
Number of pages7
JournalJournal of Physical Chemistry Letters
Volume11
Issue number11
DOIs
StatePublished - Jun 4 2020

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Cryogenic "iodide-Tagging" Photoelectron Spectroscopy: A Sensitive Probe for Specific Binding Sites of Amino Acids'. Together they form a unique fingerprint.

  • Cite this