Cryobiophysical parameters for freshly isolated human hepatocytes and HEP G2/C3A cells

A. Hubel, T. B. Darr

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


The purpose of this investigation is to determine basic cryobiophysical parameters for human and hepatoma cells. Each cell type was frozen under carefully controlled conditions and the freezing response observed. The hydraulic permeability for human hepatocytes and its activation energy was measured to be 1.6 (10)-13 m(Pa S)-1 and 2.4 (10)5 J/mol respectively. Internal ice formation parameters for human hepatocyte and hepatoma cells were estimated and compared to those of other species. These cryobiophysical parameters are useful for not only development of cryopreservation protocols but for understanding cell specific freezing characteristics.

Original languageEnglish (US)
Pages (from-to)73-74
Number of pages2
JournalAmerican Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD
StatePublished - Dec 1 1995
EventProceedings of the 1995 ASME International Mechanical Engineering Congress and Exposition - San Francisco, CA, USA
Duration: Nov 12 1995Nov 17 1995


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