Cry1Ac resistance allele frequency in field populations of Helicoverpa armigera (Hübner) collected in Telangana and Andhra Pradesh, India

Vinod S. Kukanur; T. V.K. Singh; Keshav R. Kranthi; David A. Andow

doi:10.1016/j.cropro.2018.01.008

Cry1Ac resistance allele frequency in field populations of Helicoverpa armigera (Hübner) collected in Telangana and Andhra Pradesh, India

Vinod S. Kukanur, T. V.K. Singh, Keshav R. Kranthi, David A. Andow

Entomology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Bt cotton expressing Cry1Ac insecticidal protein was introduced in India in 2002. Within a span of 12 years, Bt-cotton constituted more than 90% of cropping area covering 11.6 mHa. This large scale cultivation favours resistance evolution in target pests. One of the major factors that influences rate of resistance is frequency of resistance alleles. Using F₂ screen methodology and a discriminating dose, we estimated the level of resistance to Cry1Ac toxin in field populations of Helicoverpa armigera (Hübner). We report an increase in Cry1Ac resistance allele frequency in field populations of H. armigera collected in Telangana and Andhra Pradesh states of India. Collective allele frequency was 0.085 (95% CI) for 2013 and 0.035 (95% CI) for 2014, which was 65 times and 29 times higher than the initial frequencies estimated during 2004. To prevent further increase in Bt resistance frequency in H. armigera, it is necessary to integrate other resistance management tactics that preserves pest susceptibility to Bt cotton.

Original language	English (US)
Pages (from-to)	34-40
Number of pages	7
Journal	Crop Protection
Volume	107
DOIs	https://doi.org/10.1016/j.cropro.2018.01.008
State	Published - May 2018

Bibliographical note

Funding Information:
Special thanks to Dr. J Bentur ( Agri-Biotech Foundation, Hyderabad, India ) for valuable comments and suggestions for improving this manuscript. Sincere thanks to Indian Council of Agricultural Research (ICAR) New Delhi, India for granting fellowship to pursue Ph.D to the first author.

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

Bt resistance
Cry1Ac toxin
F screen
Helicoverpa armigera

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10.1016/j.cropro.2018.01.008

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title = "Cry1Ac resistance allele frequency in field populations of Helicoverpa armigera (H{\"u}bner) collected in Telangana and Andhra Pradesh, India",

abstract = "Bt cotton expressing Cry1Ac insecticidal protein was introduced in India in 2002. Within a span of 12 years, Bt-cotton constituted more than 90% of cropping area covering 11.6 mHa. This large scale cultivation favours resistance evolution in target pests. One of the major factors that influences rate of resistance is frequency of resistance alleles. Using F2 screen methodology and a discriminating dose, we estimated the level of resistance to Cry1Ac toxin in field populations of Helicoverpa armigera (H{\"u}bner). We report an increase in Cry1Ac resistance allele frequency in field populations of H. armigera collected in Telangana and Andhra Pradesh states of India. Collective allele frequency was 0.085 (95% CI) for 2013 and 0.035 (95% CI) for 2014, which was 65 times and 29 times higher than the initial frequencies estimated during 2004. To prevent further increase in Bt resistance frequency in H. armigera, it is necessary to integrate other resistance management tactics that preserves pest susceptibility to Bt cotton.",

keywords = "Bt resistance, Cry1Ac toxin, F screen, Helicoverpa armigera",

author = "Kukanur, {Vinod S.} and Singh, {T. V.K.} and Kranthi, {Keshav R.} and Andow, {David A.}",

note = "Funding Information: Special thanks to Dr. J Bentur ( Agri-Biotech Foundation, Hyderabad, India ) for valuable comments and suggestions for improving this manuscript. Sincere thanks to Indian Council of Agricultural Research (ICAR) New Delhi, India for granting fellowship to pursue Ph.D to the first author. Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

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doi = "10.1016/j.cropro.2018.01.008",

language = "English (US)",

volume = "107",

pages = "34--40",

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T1 - Cry1Ac resistance allele frequency in field populations of Helicoverpa armigera (Hübner) collected in Telangana and Andhra Pradesh, India

AU - Kukanur, Vinod S.

AU - Singh, T. V.K.

AU - Kranthi, Keshav R.

AU - Andow, David A.

N1 - Funding Information: Special thanks to Dr. J Bentur ( Agri-Biotech Foundation, Hyderabad, India ) for valuable comments and suggestions for improving this manuscript. Sincere thanks to Indian Council of Agricultural Research (ICAR) New Delhi, India for granting fellowship to pursue Ph.D to the first author. Publisher Copyright: © 2018 Elsevier Ltd

PY - 2018/5

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N2 - Bt cotton expressing Cry1Ac insecticidal protein was introduced in India in 2002. Within a span of 12 years, Bt-cotton constituted more than 90% of cropping area covering 11.6 mHa. This large scale cultivation favours resistance evolution in target pests. One of the major factors that influences rate of resistance is frequency of resistance alleles. Using F2 screen methodology and a discriminating dose, we estimated the level of resistance to Cry1Ac toxin in field populations of Helicoverpa armigera (Hübner). We report an increase in Cry1Ac resistance allele frequency in field populations of H. armigera collected in Telangana and Andhra Pradesh states of India. Collective allele frequency was 0.085 (95% CI) for 2013 and 0.035 (95% CI) for 2014, which was 65 times and 29 times higher than the initial frequencies estimated during 2004. To prevent further increase in Bt resistance frequency in H. armigera, it is necessary to integrate other resistance management tactics that preserves pest susceptibility to Bt cotton.

AB - Bt cotton expressing Cry1Ac insecticidal protein was introduced in India in 2002. Within a span of 12 years, Bt-cotton constituted more than 90% of cropping area covering 11.6 mHa. This large scale cultivation favours resistance evolution in target pests. One of the major factors that influences rate of resistance is frequency of resistance alleles. Using F2 screen methodology and a discriminating dose, we estimated the level of resistance to Cry1Ac toxin in field populations of Helicoverpa armigera (Hübner). We report an increase in Cry1Ac resistance allele frequency in field populations of H. armigera collected in Telangana and Andhra Pradesh states of India. Collective allele frequency was 0.085 (95% CI) for 2013 and 0.035 (95% CI) for 2014, which was 65 times and 29 times higher than the initial frequencies estimated during 2004. To prevent further increase in Bt resistance frequency in H. armigera, it is necessary to integrate other resistance management tactics that preserves pest susceptibility to Bt cotton.

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KW - Cry1Ac toxin

KW - F screen

KW - Helicoverpa armigera

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Cry1Ac resistance allele frequency in field populations of Helicoverpa armigera (Hübner) collected in Telangana and Andhra Pradesh, India

Abstract

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