Crude extract of Rheum palmatum inhibits migration and invasion of U-2 OS human osteosarcoma cells by suppression of matrix metalloproteinase-2 and -9

Shu Chun Hsu, Ju Hwa Lin, Shu Wen Weng, Fu Shin Chueh, Chien Chih Yu, Kung Wen Lu, W. Gibson Wood, Jing Gung Chung

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Osteosarcoma is the most common primary bone malignancy and primarily occurs in adolescents and young adults. Crude extract of Rheum palmatum L. (CERP) has been used as a traditional Chinese medicine for different diseases and there is experimental evidence that it may have anti-cancer effects. However, there is no information showing that CERP can affect the mobility of human osteosarcoma cells. In this study we determined the effects of CERP on U-2 OS human osteosarcoma cells. We found that CERP significantly inhibited the migration and invasion of U-2 OS cells. CERP also reduced the activity of matrix metalloproteinase (MMP)-2 and MMP-9 and decreased expression levels of the proteins FAK, GRP78, PKC, HIF-1, SOS1, VEGF, PI3K, GRB2, Ras, p-ERK1/2, ERK1/2, p-p38, JNK1/2, p-JNK1/2, MEKK3, MKK7, PERK, p-PERK, iNOS, COX-2, NF-κB p65, IRE-1, UPA, and RhoA in U-2 OS cells. Confocal laser microscopy revealed that CERP decreased the expression of NF-κB p65, RhoA and Rock 1. These invitro studies suggest that CERP may have novel anti-cancer actions in the treatment of osteosarcoma. Further studies including animal models of bone cancer are warranted.

Original languageEnglish (US)
Pages (from-to)120-129
Number of pages10
JournalBioMedicine (Netherlands)
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2013

Keywords

  • Crude extract of Rheum palmatum
  • Invasion
  • Migration
  • U-2 OS human osteosarcoma cells

Fingerprint Dive into the research topics of 'Crude extract of Rheum palmatum inhibits migration and invasion of U-2 OS human osteosarcoma cells by suppression of matrix metalloproteinase-2 and -9'. Together they form a unique fingerprint.

  • Cite this