Cross-linked DNA: Propargylated ribonucleosides as "click" ligation sites for bifunctional azides

Suresh S. Pujari, Frank Seela

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

2′-O or 3′-O-propargylated adenosines and ribothymidines were used as click targets for cross-linking of oligonucleotides with aliphatic and aromatic azides. The cross-link generates a sugar modification at the 2′-O-ligation site. Inexpensive ribonucleosides were used as starting materials. Cross-linking of oligonucleotides was performed at internal or terminal positions. Hybridization of homodimers with two complementary single strands resulted in stable ligated DNA duplexes.

Original languageEnglish (US)
Pages (from-to)4460-4465
Number of pages6
JournalJournal of Organic Chemistry
Volume77
Issue number9
DOIs
StatePublished - May 4 2012

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