Cross-feeding modulates antibiotic tolerance in bacterial communities

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34 Scopus citations

Abstract

Microbes frequently rely on metabolites excreted by other bacterial species, but little is known about how this cross-feeding influences the effect of antibiotics. We hypothesized that when species rely on each other for essential metabolites, the minimum inhibitory concentration (MIC) for all species will drop to that of the “weakest link”—the species least resistant in monoculture. We tested this hypothesis in an obligate cross-feeding system that was engineered between Escherichia coli, Salmonella enterica, and Methylobacterium extorquens. The effect of tetracycline and ampicillin were tested on both liquid and solid media. In all cases, resistant species were inhibited at significantly lower antibiotic concentrations in the cross-feeding community than in monoculture or a competitive community. However, deviation from the “weakest link” hypothesis was also observed in cross-feeding communities apparently as result of changes in the timing of growth and cross-protection. Comparable results were also observed in a clinically relevant system involving facultative cross-feeding between Pseudomonas aeruginosa and an anaerobic consortium found in the lungs of cystic fibrosis patients. P. aeruginosa was inhibited by lower concentrations of ampicillin when cross-feeding than when grown in isolation. These results suggest that cross-feeding significantly alters tolerance to antibiotics in a variety of systems.

Original languageEnglish (US)
Pages (from-to)2723-2735
Number of pages13
JournalISME Journal
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2018

Bibliographical note

Funding Information:
Acknowledgements We thank Michaela Muza for help with data collection; Jeremy Chacón for help with image analysis; and Lisa Fazzino, Sarah Hammarlund, and Ross Carlson for useful comments on the manuscript. We also thank Grant Barthel and John Oja at the University Imaging Centers for their training and help with the microscopy work. This research was funded by Natural Sciences and Engineering Research Council of Canada (PGSD2 – 487305 – 2016) and the National Institutes of Health (GM121498-01A1).

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