CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

Kanut Laoharawee; Matthew J Johnson; Branden S Moriarity

doi:10.1007/978-1-0716-0290-4_24

CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

Research output: Contribution to journal › Article › peer-review

Abstract

The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

Original language	English (US)
Pages (from-to)	435-444
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2115
DOIs	https://doi.org/10.1007/978-1-0716-0290-4_24
State	Published - Feb 1 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.

PubMed: MeSH publication types

Journal Article

Publisher link

10.1007/978-1-0716-0290-4_24

Find It

Find It at U of M Libraries

Cite this

@article{65eba0a3967e4169990cdbab1efafddd,

title = "CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells",

abstract = "The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90\% and integration rates greater than 60\% can be achieved with this method.",

author = "Kanut Laoharawee and Johnson, \{Matthew J\} and Moriarity, \{Branden S\}",

note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2020",

month = feb,

day = "1",

doi = "10.1007/978-1-0716-0290-4\_24",

language = "English (US)",

volume = "2115",

pages = "435--444",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

AU - Laoharawee, Kanut

AU - Johnson, Matthew J

AU - Moriarity, Branden S

PY - 2020/2/1

Y1 - 2020/2/1

N2 - The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

AB - The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

U2 - 10.1007/978-1-0716-0290-4_24

DO - 10.1007/978-1-0716-0290-4_24

M3 - Article

C2 - 32006415

SN - 1064-3745

VL - 2115

SP - 435

EP - 444

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

Abstract

Bibliographical note

PubMed: MeSH publication types

Publisher link

Find It

Fingerprint

Cite this