CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

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The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

Original languageEnglish (US)
Pages (from-to)435-444
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - Feb 1 2020


PubMed: MeSH publication types

  • Journal Article

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