The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.
Bibliographical notePublisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.
PubMed: MeSH publication types
- Journal Article