CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Since the advent of large-scale, detailed descriptive cancer genomics studies at the beginning of the century, such as The Cancer Genome Atlas (TCGA), labs around the world have been working to make this data useful. Data like these can be made more useful by comparison with complementary functional genomic data. One new example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Such screens can reveal genome-wide suppressors of tumorigenesis and metastasis. Here we describe the use of widely available lentiviral libraries for such screens in cultured cell lines.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages137-144
Number of pages8
Volume1907
DOIs
StatePublished - 2019

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • CRISPR/Cas9
  • Cancer
  • Genetic screen
  • Lentiviral library
  • Transformation
  • Neoplasm Proteins/antagonists & inhibitors
  • Humans
  • CRISPR-Cas Systems
  • Genome, Human
  • Genomics/methods
  • Quantitative Trait Loci
  • Neoplasms/genetics

PubMed: MeSH publication types

  • Journal Article

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