CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

Nicholas J. Slipek, Jo Varshney, David A Largaespada

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Since the advent of large-scale, detailed descriptive cancer genomics studies at the beginning of the century, such as The Cancer Genome Atlas (TCGA), labs around the world have been working to make this data useful. Data like these can be made more useful by comparison with complementary functional genomic data. One new example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Such screens can reveal genome-wide suppressors of tumorigenesis and metastasis. Here we describe the use of widely available lentiviral libraries for such screens in cultured cell lines.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages137-144
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1907
ISSN (Print)1064-3745

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Clustered Regularly Interspaced Short Palindromic Repeats
Genome
Cell Line
Atlases
Genomics
Early Detection of Cancer
Libraries
Cultured Cells
Neoplasms
Carcinogenesis
Neoplasm Metastasis

Keywords

  • CRISPR/Cas9
  • Cancer
  • Genetic screen
  • Lentiviral library
  • Transformation

Cite this

Slipek, N. J., Varshney, J., & Largaespada, D. A. (2019). CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits. In Methods in Molecular Biology (pp. 137-144). (Methods in Molecular Biology; Vol. 1907). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8967-6_11

CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits. / Slipek, Nicholas J.; Varshney, Jo; Largaespada, David A.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 137-144 (Methods in Molecular Biology; Vol. 1907).

Research output: Chapter in Book/Report/Conference proceedingChapter

Slipek, NJ, Varshney, J & Largaespada, DA 2019, CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1907, Humana Press Inc., pp. 137-144. https://doi.org/10.1007/978-1-4939-8967-6_11
Slipek NJ, Varshney J, Largaespada DA. CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 137-144. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8967-6_11
Slipek, Nicholas J. ; Varshney, Jo ; Largaespada, David A. / CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 137-144 (Methods in Molecular Biology).
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