CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Keyphrases

• Adenosine 100%
• Primary Cells 100%
• Cas9 Protein 100%
• Base Editing 100%
• Cytidine 100%
• Splice Site 100%
• Single Guide RNA (sgRNA) 100%
• High Efficiency 100%
• Immortalized Cell 100%
• T Cells 50%
• Design Tools 50%
• Targeted Gene 50%
• MHC Class I 50%
• Modular Design 50%
• Gene Disruption 50%
• Premature Stop Codon 50%
• TCR-CD3 50%
• Immune Synapse 50%
• Ensembl 50%
• Splice Acceptor 50%
• Annotated Genomes 50%
• Depth Comparison 50%
• ABE8e 50%
• Splicer 50%
• Modular Tools 50%
• Double-strand Breaks 50%
• Base Editor 50%

Biochemistry, Genetics and Molecular Biology

• CRISPR/Cas9 100%
• Adenosine 100%
• Cytidine 100%
• MHC Class I 50%
• T Cell Receptor 50%
• Stop Codon 50%
• Gene Targeting 50%
• Synapse 50%
• Gene Disruption 50%
• T Cell 50%

Immunology and Microbiology

• CRISPR/Cas9 100%
• T Cell Receptor 50%
• Synapse 50%
• MHC Class I 50%
• Gene Disruption 50%
• T Cell 50%
• Gene Targeting 50%
• Stop Codon 50%
• CD3 Antigen 50%