CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes

J. Armando Casas-Mollano; Matthew H Zinselmeier; Samuel E. Erickson; Michael J. Smanski

doi:10.1089/crispr.2020.0064

CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes

J. Armando Casas-Mollano, Matthew H Zinselmeier, Samuel E. Erickson, Michael J. Smanski

Research output: Contribution to journal › Review article › peer-review

14 Scopus citations

Abstract

CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.

Original language	English (US)
Pages (from-to)	350-364
Number of pages	15
Journal	CRISPR Journal
Volume	3
Issue number	5
DOIs	https://doi.org/10.1089/crispr.2020.0064
State	Published - Oct 1 2020

Bibliographical note

Funding Information:
J.A.C.M. and M.J.S. are part of a team supported by the Advanced Plant Technologies program, DARPA Award HR001118C0146. M.H.Z. is supported by an NIH NIGMS Biotechnology Training grant NIHT32GM008347.

Publisher Copyright:
© Copyright 2020, Mary Ann Liebert, Inc.

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abstract = "CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.",

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AU - Erickson, Samuel E.

AU - Smanski, Michael J.

N1 - Funding Information: J.A.C.M. and M.J.S. are part of a team supported by the Advanced Plant Technologies program, DARPA Award HR001118C0146. M.H.Z. is supported by an NIH NIGMS Biotechnology Training grant NIHT32GM008347. Publisher Copyright: © Copyright 2020, Mary Ann Liebert, Inc.

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N2 - CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.

AB - CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.

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CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes

Abstract

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