CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes

J. Armando Casas-Mollano, Matthew H. Zinselmeier, Samuel E. Erickson, Michael J. Smanski

Research output: Contribution to journalReview articlepeer-review

Abstract

CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.

Original languageEnglish (US)
Pages (from-to)350-364
Number of pages15
JournalCRISPR Journal
Volume3
Issue number5
DOIs
StatePublished - Oct 1 2020

Bibliographical note

Funding Information:
J.A.C.M. and M.J.S. are part of a team supported by the Advanced Plant Technologies program, DARPA Award HR001118C0146. M.H.Z. is supported by an NIH NIGMS Biotechnology Training grant NIHT32GM008347.

Publisher Copyright:
© Copyright 2020, Mary Ann Liebert, Inc.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural
  • Review

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