Crenarchaeota in Lake Michigan sediment

Barbara J. MacGregor, Duane P. Moser, Elizabeth Wheeler Alm, Kenneth H. Nealson, David A. Stahl

Research output: Contribution to journalArticlepeer-review

181 Scopus citations

Abstract

RNA from Lake Michigan sediment was hybridized with a DNA probe for archaeal 16S rRNA. There was a peak of archaeal rRNA abundance in the oxic zone and another immediately below it. Six contributing species were identified by PCR amplification of extracted DNA with primers specific for archaeal rDNA: two related to Methanosarcina acetivorans and four related to marine crenarchaeotal sequences. rRNA quantification using a DNA probe specific for this crenarchaeotal assemblage showed it is most abundant in the oxic zone, where it accounts for about 10% of total acehaeal rRNA.

Original languageEnglish (US)
Pages (from-to)1178-1181
Number of pages4
JournalApplied and environmental microbiology
Volume63
Issue number3
DOIs
StatePublished - Mar 1997

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