Creation of monosomic derivatives of human cultured cell lines

Duncan J. Clarke, Juan F. Giménez-Abián, Holger Tönnies, Heidemarie Neitzel, Karl Sperling, C. Stephen Downes, Robert T. Johnson

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Monosomic mammalian cell lines would be ideal for studying gene dosage effects, including gene imprinting, and for systematic isolation of recessive somatic mutants parallel to the invaluable mutants derived from haploid yeast. But autosomal monosomies are lethal in early development; although monosomies appear in tumors, deriving cell lines from these tumors is difficult and cannot provide several syngenic lines. We have developed a strategy for generating stable monosomic human cells, based on random autosomal Integration of the gpt plasmid, partial inhibition of DNA topoisomerase II during mitosis to promote chromatid non- disjunction, and selection against retention of gpt. These are likely to be valuable as a source of otherwise inaccessible mutants. The strategy can also be used to generate partial mammalian monosomies, which are desirable as a source of information on recessive genes and gene imprinting.

Original languageEnglish (US)
Pages (from-to)167-171
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number1
StatePublished - Jan 6 1998


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