TY - JOUR
T1 - Creating and screening Cochliobolus heterostrophus non-ribosomal peptide synthetase mutants
AU - Gillian Turgeon, B.
AU - Oide, Shinichi
AU - Bushley, Kathryn
PY - 2008/2
Y1 - 2008/2
N2 - An exhaustive characterization of the set of non-ribosomal peptide synthetase (NRPS) genes of the corn pathogen, Cochliobolus heterostrophus, and the small molecule peptides produced by the enzymes they encode, has been undertaken to ascertain the role of the peptide metabolites in the fungal cell. To date, the NRPS method of peptide biosynthesis has been described for filamentous ascomycete fungi (and to a limited extent, for basidiomycete fungi) and for bacteria, only. In addition to structural diversity, non-ribosomal peptides have a broad spectrum of biological activities, many are useful in medicine, agriculture, industry, and biological research. However, to suggest that inter-organismal activities is their primary function is likely incorrect; in fact, the physiological significance of these peptides to the producing fungi is largely unknown. We document that NRPS enzymes are purveyors of small molecules for both basal metabolism and for specialized environmental niches and that some are conserved, but most are not.
AB - An exhaustive characterization of the set of non-ribosomal peptide synthetase (NRPS) genes of the corn pathogen, Cochliobolus heterostrophus, and the small molecule peptides produced by the enzymes they encode, has been undertaken to ascertain the role of the peptide metabolites in the fungal cell. To date, the NRPS method of peptide biosynthesis has been described for filamentous ascomycete fungi (and to a limited extent, for basidiomycete fungi) and for bacteria, only. In addition to structural diversity, non-ribosomal peptides have a broad spectrum of biological activities, many are useful in medicine, agriculture, industry, and biological research. However, to suggest that inter-organismal activities is their primary function is likely incorrect; in fact, the physiological significance of these peptides to the producing fungi is largely unknown. We document that NRPS enzymes are purveyors of small molecules for both basal metabolism and for specialized environmental niches and that some are conserved, but most are not.
KW - Ascomycete
KW - Development
KW - NRPS
KW - Secondary metabolism
UR - http://www.scopus.com/inward/record.url?scp=39749187179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39749187179&partnerID=8YFLogxK
U2 - 10.1016/j.mycres.2007.10.012
DO - 10.1016/j.mycres.2007.10.012
M3 - Article
C2 - 18280721
AN - SCOPUS:39749187179
VL - 112
SP - 200
EP - 206
JO - Mycological Research
JF - Mycological Research
SN - 1878-6146
IS - 2
ER -