Creatine kinase B is necessary to limit myoblast fusion during myogenesis

Adriana Simionescu-Bankston, Christophe Pichavant, James P. Canner, Luciano H. Apponi, Yanru Wang, Craig Steeds, John T. Olthoff, Joseph J. Belanto, James M. Ervasti, Grace K. Pavlath

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Myoblast fusion is critical for proper muscle growth and regeneration. During myoblast fusion, the localization of some molecules is spatially restricted; however, the exact reason for such localization is unknown. Creatine kinase B (CKB), which replenishes local ATP pools, localizes near the ends of cultured primary mouse myotubes. To gain insights into the function of CKB, we performed a yeast two-hybrid screen to identify CKB-interacting proteins. We identified molecules with a broad diversity of roles, including actin polymerization, intracellular protein trafficking, and alternative splicing, as well as sarcomeric components. In-depth studies of α-skeletal actin and α-cardiac actin, two predominant muscle actin isoforms, demonstrated their biochemical interaction and partial colocalization with CKB near the ends of myotubes in vitro. In contrast to other cell types, specific knockdown of CKB did not grossly affect actin polymerization in myotubes, suggesting other muscle-specific roles for CKB. Interestingly, knockdown of CKB resulted in significantly increased myoblast fusion and myotube size in vitro, whereas knockdown of creatine kinase M had no effect on these myogenic parameters. Our results suggest that localized CKB plays a key role in myotube formation by limiting myoblast fusion during myogenesis.

Original languageEnglish (US)
Pages (from-to)C919-C931
JournalAmerican Journal of Physiology - Cell Physiology
Issue number11
StatePublished - Jun 1 2015

Bibliographical note

Publisher Copyright:
© 2015, The American Physiological Society.


  • Actin
  • Adenosine triphosphate
  • Muscle growth
  • Myocyte
  • Myotube


Dive into the research topics of 'Creatine kinase B is necessary to limit myoblast fusion during myogenesis'. Together they form a unique fingerprint.

Cite this