Coupling chiral homogeneous biocatalytic reactions with benign heterogeneous separation

Elizabeth M. Hill, James M. Broering, Jason P. Hallett, Andreas S. Bommarius, Charles L. Liotta, Charles A. Eckert

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We describe a method for sustainable biocatalysis in an organic aqueous tunable solvent (OATS) system in which a hydrophobic substrate is transformed with a homogeneous enzymatic catalyst in a single liquid phase. Subsequent CO2 addition produces a biphasic mixture where the hydrophobic product partitions preferentially into the organic rich phase for separation while the hydrophilic enzyme catalyst partitions into the aqueous rich phase, where it is recyclable. Greater than 99% enantiomeric excess (ee) is shown for catalyzed hydrolysis of rac-1-phenylethyl acetate with Candida antarctica lipase B (CAL B) both before and after CO2-induced separation. Additionally, processing parameters in OATS systems are discussed. This system combines homogeneous enzymatic reactions with a built-in heterogeneous separation for enantiomerically pure products.

Original languageEnglish (US)
Pages (from-to)888-889
Number of pages2
JournalGreen Chemistry
Issue number8
StatePublished - Aug 1 2007


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