Correlative transmission and scanning electron microscopy study of microglia activated by interferon-,y and tumor necrosis factor-cx in vitro

W. Robert Anderson, Alice Martella, Zachary M. Drake, Shuxian Hu, Phillip K. Peterson, Chun C. Chao

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Microglia, in response to cytokines, demonstrate a number o f enhanced biochemical and functional properties which reflect a state o f activation. In this study, we evaluated the ultrastructural alterations o f murine microglia that were associated with activation by interferon-gamma (IFN-y) plus tumor necrosis factor-alpha (TNF-a). Microglial cell culture treated with these cytokines generated significant amounts o f the free radical nitric oxide (NO), a biochemical marker o f activation. Correlative transmission (TEM) and scanning (SEM) electron microscopic analyses o f these cytokine-activated microglia demonstrated two prominent features: proliferation o f cell processes and increased formation o f membrane bound dense bodies typical o f lysosomes. Since activated microglia have been implicated in the pathogenesis o f a number o f neurodegenerative diseases, application o f the ultrastructural findings in the in vitro study may prove useful in determining the state o f activation o f microglia in brain specimens from patients with these neurological disorders.

Original languageEnglish (US)
Pages (from-to)1016-1022
Number of pages7
JournalPathology Research and Practice
Volume191
Issue number10
DOIs
StatePublished - 1995

Keywords

  • Central nervous system
  • Cytokine
  • IrnmuneactivationInterferon
  • Lysosornes
  • Microglia
  • Neurodegenerative disease
  • Nitric oxide
  • Scanningelectronmicroscopy
  • Transmission electron microscopy
  • Tumor necrosis factor-α

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