TY - JOUR
T1 - Correlations between pathogen concentration and fecal indicator marker genes in beach environments
AU - Zhang, Qian
AU - Eichmiller, Jessica J.
AU - Staley, Christopher
AU - Sadowsky, Michael J.
AU - Ishii, Satoshi
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/12/15
Y1 - 2016/12/15
N2 - The concentrations of several potential pathogens were measured using a microfluidic quantitative PCR (MFQPCR) platform in beach water, sand, and sediment samples collected in Duluth, MN. Among the 19 pathogen marker genes examined, eaeA from Escherichia coli and plc from Clostridium perfringens were most frequently detected in all samples. In beach water and wastewater samples, positive correlations were observed between quantities of potential pathogens and most of the fecal indicator genetic markers. Such correlations, however, were not frequently observed in sand and sediment samples. Our results suggest that the behavior of potential pathogens and FIB may vary by sample type and source of contamination. Consequently, appropriate FIB marker genes need to be chosen for reliable water/sand quality monitoring.
AB - The concentrations of several potential pathogens were measured using a microfluidic quantitative PCR (MFQPCR) platform in beach water, sand, and sediment samples collected in Duluth, MN. Among the 19 pathogen marker genes examined, eaeA from Escherichia coli and plc from Clostridium perfringens were most frequently detected in all samples. In beach water and wastewater samples, positive correlations were observed between quantities of potential pathogens and most of the fecal indicator genetic markers. Such correlations, however, were not frequently observed in sand and sediment samples. Our results suggest that the behavior of potential pathogens and FIB may vary by sample type and source of contamination. Consequently, appropriate FIB marker genes need to be chosen for reliable water/sand quality monitoring.
KW - Fecal indicator
KW - Genetic markers
KW - Pathogens
KW - Quantitative PCR
KW - Water quality
UR - http://www.scopus.com/inward/record.url?scp=84984861310&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84984861310&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2016.08.122
DO - 10.1016/j.scitotenv.2016.08.122
M3 - Article
C2 - 27595940
AN - SCOPUS:84984861310
SN - 0048-9697
VL - 573
SP - 826
EP - 830
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -
