Correlation between synovial fluid cytokine concentrations and limb function in normal dogs and in dogs with lameness from spontaneous osteoarthritis

Philip I. Allen, Michael G Conzemius, Richard B. Evans, Kristina Kiefer

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Objective: To determine the relationship between synovial biomarker concentrations and severity of lameness and to assess the ability to differentiate normal from osteoarthritic joints with synovial biomarker concentrations. Study design: Prospective clinical study. Sample population: Twelve hounds with no evidence of osteoarthritis (OA) and 27 client-owned dogs with unilateral lameness and joint pain in a single joint from naturally occurring OA. Methods: Enrollment in the OA group required a history of lameness, radiographic evidence of OA on orthogonal joint radiographs, and ≥6% gait asymmetry between contralateral limbs. The concentrations of 14 synovial OA biomarkers in synovial samples obtained after gait analysis were measured with enzyme-linked immunosorbent assays and compared between normal and OA joints. Results: Concentrations of monocyte chemoattractant protein (MCP)-1, substance P, interleukin (IL)-6, IL-8, KC-like, matrix metalloproteinase (MMP)-1, and MMP-3 were greater (P ≤.05) in OA than in normal joints. The concentrations of bradykinin and tissue inhibitors of metalloproteinase-4 were decreased in OA compared with normal joints. Monocyte chemoattractant protein 1 was identified as the most accurate marker to distinguish OA from normal joints. No correlation was detected between any OA biomarker concentration, individually or in combination, and severity of gait asymmetry at the walk. Conclusion: Differences in proinflammatory and anti-inflammatory biomarkers were detected between OA and normal joints, but no relationship was identified between biomarker concentrations and gait asymmetry in dogs with OA. Clinical impact: This information will help guide future studies to elucidate how factors such as disease chronicity, severity, and etiology affect these relationships.

Original languageEnglish (US)
Pages (from-to)770-779
Number of pages10
JournalVeterinary Surgery
Issue number5
StatePublished - Jul 2019

Bibliographical note

Funding Information:
information Tata Endowment; University of Minnesota Small Companion Animal Grant, Grant/Award Number: 507; Tata Group Endowment; University of Minnesota Department of Clinical Science Small Companion Animal Grant The authors thank Donna Groschen BS, CVT and Sara Pracht BA, CVT for their help and technical expertise.

Publisher Copyright:
© 2019 The American College of Veterinary Surgeons


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