Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency

Deborah A Ferrington, Heidi Roehrich, Angela A. Chang, Craig W. Huang, Marcela Maldonado, Wendy Bratten, Abrar A. Rageh, Neal D Heuss, Dale S Gregerson, Elizabeth F. Nelson, Ching Yuan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7-/-/mecl-1-/-, L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.

Original languageEnglish (US)
Article numbere54347
JournalPloS one
Volume8
Issue number1
DOIs
StatePublished - Jan 30 2013

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Immune system
cornea
tissue repair
Cell culture
Wound Healing
Cornea
Cells
Cytokines
homeostasis
mice
Homeostasis
Re-Epithelialization
debridement
Corneal Epithelium
Debridement
cells
protein subunits
immune system
Immune System
Catalytic Domain

Cite this

Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency. / Ferrington, Deborah A; Roehrich, Heidi; Chang, Angela A.; Huang, Craig W.; Maldonado, Marcela; Bratten, Wendy; Rageh, Abrar A.; Heuss, Neal D; Gregerson, Dale S; Nelson, Elizabeth F.; Yuan, Ching.

In: PloS one, Vol. 8, No. 1, e54347, 30.01.2013.

Research output: Contribution to journalArticle

Ferrington, DA, Roehrich, H, Chang, AA, Huang, CW, Maldonado, M, Bratten, W, Rageh, AA, Heuss, ND, Gregerson, DS, Nelson, EF & Yuan, C 2013, 'Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency', PloS one, vol. 8, no. 1, e54347. https://doi.org/10.1371/journal.pone.0054347
Ferrington, Deborah A ; Roehrich, Heidi ; Chang, Angela A. ; Huang, Craig W. ; Maldonado, Marcela ; Bratten, Wendy ; Rageh, Abrar A. ; Heuss, Neal D ; Gregerson, Dale S ; Nelson, Elizabeth F. ; Yuan, Ching. / Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency. In: PloS one. 2013 ; Vol. 8, No. 1.
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