Abstract
Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7-/-/mecl-1-/-, L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.
| Original language | English (US) |
|---|---|
| Article number | e54347 |
| Journal | PloS one |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 30 2013 |
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Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency. / Ferrington, Deborah A; Roehrich, Heidi; Chang, Angela A.; Huang, Craig W.; Maldonado, Marcela; Bratten, Wendy; Rageh, Abrar A.; Heuss, Neal D; Gregerson, Dale S; Nelson, Elizabeth F.; Yuan, Ching.
In: PloS one, Vol. 8, No. 1, e54347, 30.01.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Corneal Wound Healing Is Compromised by Immunoproteasome Deficiency
AU - Ferrington, Deborah A
AU - Roehrich, Heidi
AU - Chang, Angela A.
AU - Huang, Craig W.
AU - Maldonado, Marcela
AU - Bratten, Wendy
AU - Rageh, Abrar A.
AU - Heuss, Neal D
AU - Gregerson, Dale S
AU - Nelson, Elizabeth F.
AU - Yuan, Ching
PY - 2013/1/30
Y1 - 2013/1/30
N2 - Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7-/-/mecl-1-/-, L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.
AB - Recent studies have revealed roles for immunoproteasome in regulating cell processes essential for maintaining homeostasis and in responding to stress and injury. The current study investigates how the absence of immunoproteasome affects the corneal epithelium under normal and stressed conditions by comparing corneas from wildtype (WT) mice and those deficient in two immunoproteasome catalytic subunits (lmp7-/-/mecl-1-/-, L7M1). Immunoproteasome expression was confirmed in WT epithelial cells and in cells of the immune system that were present in the cornea. More apoptotic cells were found in both corneal explant cultures and uninjured corneas of L7M1 compared to WT mice. Following mechanical debridement, L7M1 corneas displayed delayed wound healing, including delayed re-epithelialization and re-establishment of the epithelial barrier, as well as altered inflammatory cytokine production compared to WT mice. These results suggest that immunoproteasome plays an important role in corneal homeostasis and wound healing.
UR - http://www.scopus.com/inward/record.url?scp=84872842402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872842402&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0054347
DO - 10.1371/journal.pone.0054347
M3 - Article
C2 - 23365662
AN - SCOPUS:84872842402
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 1
M1 - e54347
ER -