Coordinated regulation of growth genes in Saccharomyces cerevisiae

Matthew G. Slattery, Warren Heideman

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


It is imperative that quiescent Saccharomyces cerevisiae cells respond rapidly to fresh medium: the cell that initiates growth and division soonest has the most progeny. Several laboratories have used DNA microarrays to identify transcripts that are altered when fresh medium is added to quiescent cells. We combined published data with our own to address several questions: Do these experiments taken together identify a core set of genes that is reproducibly affected when quiescent cells are stimulated by nutrient repletion? Is this gene set coregulated in response to other environmental challenges? Does promoter histone occupancy correlate with the mRNA data? Despite diverse experimental designs, the data were highly correlated, generating a set of nutrient repletion transcripts. Glucose addition accounted for the response. These transcripts were also coregulated in response to diverse stresses. Promoters were associated with increased histone acetylation and decreased histone occupancy when induced, and high histone occupancy with low acetylation when repressed. The presence of RRPE and PAC promoter elements correlated with nutrient responsiveness and a dynamic pattern of histone occupancy and acetylation. Correlative evidence supports the idea that some mRNAs may be upregulated by release from sequestration in RNA-protein complexes.

Original languageEnglish (US)
Pages (from-to)1210-1219
Number of pages10
JournalCell Cycle
Issue number10
StatePublished - May 15 2007

Bibliographical note

Funding Information:
The authors wish to acknowledge Audrey Gasch, Dritan Liko, Jay Farrar, and Michael Conway for helpful discussions. We are also indebted to the researchers who worked to create the data cited. This work was supported by National Science Foundation Grant MCB-0542779 (W.H.) and predoctoral fellowship from the American Foundation for Pharmaceutical Education (M.G.S.).


  • Chromatin
  • Environmental stress
  • Gene expression
  • Glucose
  • Growth
  • Yeast


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