Hydrogen exchange experiments under slow exchange conditions show that an omega loop in cytochrome c (residues 40-57) acts as a cooperative unfolding/refolding unit under native conditions. This unit behavior accounts for an initial step on the unfolding pathway, a final step in refolding, and a number of other structural, functional and evolutionary properties.
Bibliographical noteFunding Information:
This work was supported by research grants from the NIH and the Mathers Foundation.
- Cytochrome c
- Hydrogen exchange
- Omega loop
- Protein folding
- Protein function