Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies

Melissa Weldy, Clayton Evert, Peter I Dosa, Alexander Khoruts, Michael J Sadowsky

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Clostridioides difficile, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol that can be practiced in any lab setting using a portable anaerobic glove bag. This protocol also optimizes existing cell growth methods and presents a detailed trouble shooting guide. This protocol is a modification of those previously reported by Edwards and McBride (2016) and Shen et al. (2016).

Original languageEnglish (US)
Article number100071
JournalSTAR Protocols
Volume1
Issue number2
DOIs
StatePublished - Sep 18 2020

Bibliographical note

Funding Information:
This work was supported, in part, by the Office of the Assistant Secretary of Defense for Health Affairs , under Investigator Initiated Research Awards W81XWH-17-1-0635 (P.I.D.) and W81XWH-17-1-0636 (A.K.) and by grant 5U01HL127479-03 from the NIH , under the MIN-REACH Research and Evaluation Hub. We would like to thank Alexa Weingarden and Raymond Erickson for helpful discussions and John Ferguson for help with images.

Publisher Copyright:
© 2020 The Author(s)

PubMed: MeSH publication types

  • Journal Article

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