Controlled freezing studies as a corollary selection method for winterhardiness in perennial flax

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Perennial flax (Linum spp.) breeding objectives at the University of Minnesota (UMN) are to develop agronomic (oilseed, fiber) and horticultural (cut flower, garden perennial) varieties that are hardy in Minnesota (USDA Plant Hardiness Zones 3 and 4). The objective of this research was to determine the range of cold hardiness in UMN perennial flax breeding populations compared with wild accessions of L. austriacum, L. lewisii, and L. perenne. Fifty-three genotypes from seven populations were low temperature acclimated, followed by controlled freezing using a programmable freezer. The observed lethal temperature for 50% plant kill (LT50) ranged from >0 °C (0% survival) to <−12 °C (100% survival), for the test temperatures of 0, −4, −8, −10, and −12 °C. Cold damage was measured after 4 wk of regrowth as the proportion of alive shoots and a root damage rating. Significant negative correlations were observed between LT50 and proportion of alive shoots (r = −.918) and root damage rating (r = −.935). Both L. austriacum and L. perenne had significantly less cold damage than L. lewisii and the breeding populations. This study establishes methods of screening perennial flax cold tolerance that are more cost effective, rapid, and repeatable compared to field evaluations.

Original languageEnglish (US)
Pages (from-to)1734-1757
Number of pages24
JournalCrop Science
Issue number5
StatePublished - Sep 1 2022

Bibliographical note

Funding Information:
This research was supported by the Minnesota Department of Agriculture Clean Water Fund.

Publisher Copyright:
© 2022 The Authors. Crop Science © 2022 Crop Science Society of America.


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