Abstract
Control of protein synthesis is critical for synaptic plasticity and memory formation. However, the molecular mechanisms linking neuronal activity to activation of mRNA translation are not fully understood. Here, we report that the translational repressor poly(A)-binding protein (PABP)-interacting protein 2A (PAIP2A), an inhibitor of PABP, is rapidly proteolyzed by calpains in stimulated neurons and following training for contextual memory. Paip2a knockout mice exhibit a lowered threshold for the induction of sustained long-term potentiation and an enhancement of long-term memory after weak training. Translation of CaMKIIα mRNA is enhanced in Paip2a-/- slices upon tetanic stimulation and in the hippocampus of Paip2a-/- mice following contextual fear learning. We demonstrate that activity-dependent degradation of PAIP2A relieves translational inhibition of memory-related genes through PABP reactivation and conclude that PAIP2A is a pivotal translational regulator of synaptic plasticity and memory
| Original language | English (US) |
|---|---|
| Pages (from-to) | 298-311 |
| Number of pages | 14 |
| Journal | Neuron |
| Volume | 78 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 24 2013 |
Bibliographical note
Funding Information:We thank Wayne Sossin and Valerie Henderson for excellent comments; Annie Sylvestre, Sandra Perreault, and Colin Lister for technical assistance; and Isabel Laplante for assistance with immunostaining. This work was supported by National Institutes of Health Grant 2R01GM066157 and a Canadian Institutes of Health Research (CIHR) grant to Nahum Sonenberg and CIHR Grant MOP-10848 to J.C.L. J.C.L. is the recipient of the Canada Research Chair in Cellular and Molecular Neurophysiology.