Control of Saccharomyces cerevisiae 2μm DNA replication by cell division cycle genes that control nuclear DNA replication

Dennis M. Livingston, Doris M. Kupfer

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Abstract

The replication of the 2 μm DNA of Saccharomyces cerevisiae has been examined in cell division cycle (cdc) mutants. The 2 μm DNA does not replicate at the restrictive temperature in cells bearing the cdc28, cdc4, and cdc7 mutations which prevent passage of cells from the G1 phase into S phase. Plasmid replication also is prevented in a mating-type cells by α factor, a mating hormone which prevents cells from completing an event early in G1 phase. The 2 μm DNA ceases replication at 36 °C in a mutant harboring the cdc8 mutation, a defect in the elongation reactions of nuclear DNA replication. Plasmid replication continues at the restrictive temperature for approximately one generation in a cdc13 mutant defective in nuclear division. These results show that 2 μm DNA replication is controlled by the same genes that control the initiation and completion of nuclear DNA replication.

Original languageEnglish (US)
Pages (from-to)249-260
Number of pages12
JournalJournal of Molecular Biology
Volume116
Issue number2
DOIs
StatePublished - Oct 25 1977

Bibliographical note

Funding Information:
WC thank Leland H. Hartwell for his interc~st and support of this work. This project was supported by a grant from the National Inst,itute of General Medical Sciences (GM17709) to Leland H. Hartwell. One of us (D.M.L.) is a postdoctoral fellow of the National Ca~~ccr Institllt,e (CA 00592).

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