Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis

James R Johnson; Giuseppe Magistro; Connie Clabots; Stephen Porter; Amee Manges; Paul Thuras; Sören Schubert

doi:10.1016/j.micpath.2018.04.048

Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis

James R Johnson, Giuseppe Magistro, Connie Clabots, Stephen Porter, Amee Manges, Paul Thuras, Sören Schubert

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Escherichia coli sequence type 69 (ST69; “clonal group A”) is an important extraintestinal pathogen. To clarify the yersiniabactin siderophore system's role in ST69's extraintestinal virulence we compared a wild-type ST69 cystitis isolate, isogenic irp2 (yersiniabactin) mutants, and irp2-complemented mutants in murine models of sepsis and urinary tract infection (UTI). irp2 mutants were attenuated mildly in the UTI model and profoundly in the sepsis model. In both models, complementation with a functional copy of irp2 restored full parental virulence. These findings suggest that in ST69 the yersiniabactin system has a minor role in urovirulence and a major role in sepsis causation.

Original language	English (US)
Pages (from-to)	128-131
Number of pages	4
Journal	Microbial Pathogenesis
Volume	120
DOIs	https://doi.org/10.1016/j.micpath.2018.04.048
State	Published - Jul 2018

Bibliographical note

Funding Information:
This work was supported in part by Office of Research and Development, Department of Veterans Affairs, grant # 2I01CX000920-04 (to J.R.J.). The funder had no involvement in study design or conduct, manuscript preparation, or the decision to publish. The opinions expressed here are strictly those of the authors and not those of their respective institutions or the Department of Veterans Affairs. Appendix A

Publisher Copyright:
© 2018

Keywords

Clonal group A
Escherichia coli
Sepsis
Urinary tract infection
Virulence
Yersiniabactin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micpath.2018.04.048

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Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis. / Johnson, James R; Magistro, Giuseppe; Clabots, Connie; Porter, Stephen; Manges, Amee; Thuras, Paul; Schubert, Sören.

In: Microbial Pathogenesis, Vol. 120, 07.2018, p. 128-131.

Research output: Contribution to journal › Article › peer-review

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title = "Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis",

abstract = "Escherichia coli sequence type 69 (ST69; “clonal group A”) is an important extraintestinal pathogen. To clarify the yersiniabactin siderophore system's role in ST69's extraintestinal virulence we compared a wild-type ST69 cystitis isolate, isogenic irp2 (yersiniabactin) mutants, and irp2-complemented mutants in murine models of sepsis and urinary tract infection (UTI). irp2 mutants were attenuated mildly in the UTI model and profoundly in the sepsis model. In both models, complementation with a functional copy of irp2 restored full parental virulence. These findings suggest that in ST69 the yersiniabactin system has a minor role in urovirulence and a major role in sepsis causation.",

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author = "Johnson, {James R} and Giuseppe Magistro and Connie Clabots and Stephen Porter and Amee Manges and Paul Thuras and S{\"o}ren Schubert",

note = "Funding Information: This work was supported in part by Office of Research and Development, Department of Veterans Affairs, grant # 2I01CX000920-04 (to J.R.J.). The funder had no involvement in study design or conduct, manuscript preparation, or the decision to publish. The opinions expressed here are strictly those of the authors and not those of their respective institutions or the Department of Veterans Affairs. Appendix A Publisher Copyright: {\textcopyright} 2018",

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AU - Magistro, Giuseppe

AU - Clabots, Connie

AU - Porter, Stephen

AU - Manges, Amee

AU - Thuras, Paul

AU - Schubert, Sören

N1 - Funding Information: This work was supported in part by Office of Research and Development, Department of Veterans Affairs, grant # 2I01CX000920-04 (to J.R.J.). The funder had no involvement in study design or conduct, manuscript preparation, or the decision to publish. The opinions expressed here are strictly those of the authors and not those of their respective institutions or the Department of Veterans Affairs. Appendix A Publisher Copyright: © 2018

PY - 2018/7

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N2 - Escherichia coli sequence type 69 (ST69; “clonal group A”) is an important extraintestinal pathogen. To clarify the yersiniabactin siderophore system's role in ST69's extraintestinal virulence we compared a wild-type ST69 cystitis isolate, isogenic irp2 (yersiniabactin) mutants, and irp2-complemented mutants in murine models of sepsis and urinary tract infection (UTI). irp2 mutants were attenuated mildly in the UTI model and profoundly in the sepsis model. In both models, complementation with a functional copy of irp2 restored full parental virulence. These findings suggest that in ST69 the yersiniabactin system has a minor role in urovirulence and a major role in sepsis causation.

AB - Escherichia coli sequence type 69 (ST69; “clonal group A”) is an important extraintestinal pathogen. To clarify the yersiniabactin siderophore system's role in ST69's extraintestinal virulence we compared a wild-type ST69 cystitis isolate, isogenic irp2 (yersiniabactin) mutants, and irp2-complemented mutants in murine models of sepsis and urinary tract infection (UTI). irp2 mutants were attenuated mildly in the UTI model and profoundly in the sepsis model. In both models, complementation with a functional copy of irp2 restored full parental virulence. These findings suggest that in ST69 the yersiniabactin system has a minor role in urovirulence and a major role in sepsis causation.

KW - Clonal group A

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KW - Virulence

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Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis

Abstract

Bibliographical note

Keywords

UN SDGs

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