Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans

Liangliang Gao, James M. Bradeen

Research output: Contribution to journalArticle

  • 3 Citations

Abstract

The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.

Original languageEnglish (US)
Article numbere0159969
JournalPLoS One
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2016

Fingerprint

Phytophthora infestans
Solanum tuberosum
RNA
Genes
Gene Ontology
Gene Dosage
Transcriptome
Lipid Metabolism
Cell Wall

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

MeSH PubMed subject areas

  • Journal Article

Cite this

Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans. / Gao, Liangliang; Bradeen, James M.

In: PLoS One, Vol. 11, No. 7, e0159969, 01.07.2016.

Research output: Contribution to journalArticle

Gao, Liangliang; Bradeen, James M. / Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans.

In: PLoS One, Vol. 11, No. 7, e0159969, 01.07.2016.

Research output: Contribution to journalArticle

@article{1844c6bacf2f4092b13eafde834c155d,
title = "Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans",
abstract = "The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.",
author = "Liangliang Gao and Bradeen, {James M.}",
year = "2016",
month = "7",
doi = "10.1371/journal.pone.0159969",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans

AU - Gao,Liangliang

AU - Bradeen,James M.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.

AB - The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.

UR - http://www.scopus.com/inward/record.url?scp=84979517135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979517135&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0159969

DO - 10.1371/journal.pone.0159969

M3 - Article

VL - 11

JO - PLoS One

T2 - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e0159969

ER -