Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans

Liangliang Gao, James M. Bradeen

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.

Original languageEnglish (US)
Article numbere0159969
JournalPloS one
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2016

Bibliographical note

Funding Information:
This work was funded by USDA NIFA (grant 1007-35300-18255), EPA P3 (grant SU-83434601-0), and the Minnesota Area II Potato Research and Promotion Council. We thank Dr. Benjamin P. Millet (University of Minnesota) for identifying the transgenic line SP2211, pioneering tuber resistance phenotyping and developing molecular assays for transgene transcription measurement. We thank Dr. Mark Lohse (Max Planck Institute, Germany) for generating a draft PGSC mapping file for MapMan analysis in potato. Computing resources from the University of Minnesota Supercomputing Institute are greatly appreciated.

Publisher Copyright:
© 2016 Gao, Bradeen. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Fingerprint

Dive into the research topics of 'Contrasting potato foliage and tuber defense mechanisms against the late blight pathogen Phytophthora infestans'. Together they form a unique fingerprint.

Cite this