Contrast-enhanced in vivo imaging of breast and prostate cancer cells by MRI. Rodriguez O, Fricke S, Chien C, Dettin L, VanMeter J, Shapiro E, Dai HN, Casimiro M, Ileva L, Dagata J, Johnson MD, Lisanti MP, Koretsky A, Albanese C, Lombardi Cancer Center, Department of Oncology, Georgetown University Medical Center, Washington, DC

Research output: Contribution to journalShort surveypeer-review

Abstract

The development of effective cancer therapies has been hampered, in part, by the inability to noninvasively follow tumor progression from the initial cancerous lesion through to metastasis. We have previously shown that superparamagnetic iron oxide particles can be used as magnetic resonance imaging contrast agents to label embryonic, mesenchymal, and hematopoietic stem cells in vivo. Improving the capacity to noninvasively image cancer progression is an appealing method that could be useful for assessing the efficacy of anticancer therapies. We have established that human prostate (LNCaP, DU145, PC3), rodent prostate (TRAMPC1, YPEN-1), human breast (MDA-MB-231), and mouse mammary (Myc/VEGF) cancer cell lines were readily labeled by fluorescent superparamagnetic submicron particles of iron oxide (MPIOs). The MPIOs were essentially inert with respect to cell proliferation and tumor formation. Fluorescence stereomicroscopy and three dimensional magnetic resonance imaging (MRI) determined that subcutaneous, intramuscular, or orthotopically implanted labeled cancer cells could be imaged, in vivo, despite in some cases being undetectable by manual palpation. The MPIO-labeled cancer cells could also be imaged, in vivo, at least 6 weeks after implantation. The fluorescent MPIOs further allowed for the ex vivo identification of tumors cells from histological sections. This study demonstrates the feasibility of using fluorescent MPIOs in prostate and breast cancer cell lines as both a negative contrast agent for in vivo MRI as well as a fluorescent tumor marker for optical imaging in vivo and ex vivo.

Original languageEnglish (US)
Pages (from-to)357-358
Number of pages2
JournalUrologic Oncology: Seminars and Original Investigations
Volume25
Issue number4
DOIs
StatePublished - Jul 1 2007

Fingerprint Dive into the research topics of 'Contrast-enhanced in vivo imaging of breast and prostate cancer cells by MRI. Rodriguez O, Fricke S, Chien C, Dettin L, VanMeter J, Shapiro E, Dai HN, Casimiro M, Ileva L, Dagata J, Johnson MD, Lisanti MP, Koretsky A, Albanese C, Lombardi Cancer Center, Department of Oncology, Georgetown University Medical Center, Washington, DC'. Together they form a unique fingerprint.

Cite this