Continuous fluorometric assay of phospholipase A2 with pyrene-labeled lecithin as a substrate

H. Stewart Hendrickson, Phillip N Rauk

Research output: Contribution to journalArticlepeer-review

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Abstract

1,2-Bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphorylcholine was synthesized as a fluorogenic substrate for phospholipase A2. It has a critical micellar concentration of 7.3 μm and gives only excimer fluorescent emission at 480 nm in aqueous micellar dispersion. When hydrolyzed by phospholipase A2, the products give only monomer emission which is monitored best at 382 and 400 nm. Conditions were developed for an assay for phospholipase A2 using this substrate. The assay was sensitive to as little as 8 ng of pure porcine pancreatic phospholipase A2.

Original languageEnglish (US)
Pages (from-to)553-558
Number of pages6
JournalAnalytical Biochemistry
Volume116
Issue number2
DOIs
StatePublished - Sep 15 1981
Externally publishedYes

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