@inproceedings{0c9b67b9a74546e59e1b4093af48157e,
title = "Contamination-free droplet fusion and continuous flow PCR",
abstract = "We present a new system for performing continuous flow polymerase chain reaction (PCR) using aqueous droplets carried by immiscible oil. By precisely controlling the interfacial properties, we achieve reproducible, contamination-free PCR. We also demonstrate how a template droplet can be mixed in flow with a reagent droplet using the contact-free method of electrocoalescence, which we optimized in field strength and frequency. We estimate that a fully automated version of our system with online sample preparation has the potential to perform 50,000 PCR reactions per day in droplets smaller than 100 nl.",
keywords = "Coalescence, Contamination, Droplets, PCR",
author = "M. Chabert and Dorfman, {K. D.} and Viovy, {J. L.}",
year = "2005",
month = jan,
day = "1",
language = "English (US)",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "115--117",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}