Contamination-free droplet fusion and continuous flow PCR

M. Chabert, K. D. Dorfman, J. L. Viovy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present a new system for performing continuous flow polymerase chain reaction (PCR) using aqueous droplets carried by immiscible oil. By precisely controlling the interfacial properties, we achieve reproducible, contamination-free PCR. We also demonstrate how a template droplet can be mixed in flow with a reagent droplet using the contact-free method of electrocoalescence, which we optimized in field strength and frequency. We estimate that a fully automated version of our system with online sample preparation has the potential to perform 50,000 PCR reactions per day in droplets smaller than 100 nl.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages115-117
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Coalescence
  • Contamination
  • Droplets
  • PCR

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