Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications

Kevin D. Dorfman, Max Chabert, Jean Hugues Codarbox, Gilles Rousseau, Patricia De Cremoux, Jean Louis Viovy

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

We present a method for performing polymerase chain reaction (PCR) using isolated droplets flowing in an immiscible fluorinated solvent system. Thanks to an optimized control of interfacial properties, we could achieve in this capillary-based system reproducible amplification factors, without any detectable contamination between neighboring droplets. The system is readily amenable to further miniaturization and automation and serves as the first step toward a clinically viable, high-throughput, quantitative continuous flow PCR apparatus.

Original languageEnglish (US)
Pages (from-to)3700-3704
Number of pages5
JournalAnalytical Chemistry
Volume77
Issue number11
DOIs
StatePublished - Jun 1 2005

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