Construction of large DNA segments in Escherichia coli

Michael O'Connor, Mark Peifer, Welcome Bender

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

Recombinant DNA clones containing large pieces of DNA are useful in the study of large genetic units, but these are difficult to make in most bacterial cloning vectors. A strategy is described that uses general and sitespecific recombination to construct large pieces of eukaryotic DNA from smaller cloned segments. The large clones are propagated on F factor-based plasmids in Escherichia coli. They can be easily modified to introduce mutations or rearrangements. These techniques were applied to the construction of large DNA segments from the bithorax complex of Drosophila.

Original languageEnglish (US)
Pages (from-to)1307-1312
Number of pages6
JournalScience
Volume244
Issue number4910
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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