Construction of artificial biosynthetic pathways for resveratrol glucoside derivativesS

Oksik Choi, Jae Kyoung Lee, Sun Young Kang, Ramesh Prasad Pandey, Jae Kyung Sohng, Jong Seog Ahn, Young Soo Hong

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Resveratrol, which is a polyphenolic antioxidant, is dose-dependent when used to provide health benefits, to enhance stress resistance, and to extend lifespans. However, even though resveratrol has therapeutic benefits, its clinical therapeutic effect is limited owing to its low oral bioavailability. An Escherichia coli system was developed that contains an artificial biosynthetic pathway that produces resveratrol glucoside derivatives, such as resveratrol-3-Oglucoside (piceid) and resveratrol-4'-O-glucoside (resveratroloside), from simple carbon sources. This artificial biosynthetic pathway contains a glycosyltransferase addition (YjiC from Bacillus) with resveratrol biosynthetic genes. The produced glucoside compounds were verified through the presence of a product peak(s) and also through LC/MS analyses. The strategy used in this research demonstrates the first harnessing of E. coli for de novo synthesis of resveratrol glucoside derivatives from a simple sugar medium.

Original languageEnglish (US)
Pages (from-to)614-618
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume24
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Artificial biosynthesis
  • Glycosylation
  • Piceid
  • Resveratrol

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