Construction of a DNA library on microbeads using whole genome amplification

Takaaki Kojima, Bo Zhu, Hideo Nakano

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a “bead library”, has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages87-100
Number of pages14
DOIs
StatePublished - Sep 19 2015

Publication series

NameMethods in Molecular Biology
Volume1347
ISSN (Print)1064-3745

Keywords

  • Emulsion PCR
  • Genomic library
  • Magnetic beads
  • Microdroplets

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