A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a “bead library”, has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||14|
|State||Published - Sep 19 2015|
|Name||Methods in Molecular Biology|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2015.
- Emulsion PCR
- Genomic library
- Magnetic beads