Construction of a DNA library on microbeads using whole genome amplification

Takaaki Kojima; Bo Zhu; Hideo Nakano

doi:10.1007/978-1-4939-2990-0_6

Construction of a DNA library on microbeads using whole genome amplification

Takaaki Kojima, Bo Zhu, Hideo Nakano

Biotechnology Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a “bead library”, has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	87-100
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-2990-0_6
State	Published - Sep 19 2015

Publication series

Name	Methods in Molecular Biology
Volume	1347
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Emulsion PCR
Genomic library
Magnetic beads
Microdroplets

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10.1007/978-1-4939-2990-0_6

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AU - Zhu, Bo

AU - Nakano, Hideo

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PY - 2015/9/19

Y1 - 2015/9/19

N2 - A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a “bead library”, has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.

AB - A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a “bead library”, has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.

KW - Emulsion PCR

KW - Genomic library

KW - Magnetic beads

KW - Microdroplets

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Construction of a DNA library on microbeads using whole genome amplification

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