Construction of a 2-Mb resolution BAC microarray for CGH analysis of canine tumors

Rachael Thomas, Allyson Scott, Cordelia F. Langford, Susan P. Fosmire, Cristan M. Jubala, Travis D. Lorentzen, Christophe Hitte, Elinor K. Karlsson, Ewen Kirkness, Elaine A. Ostrander, Francis Galibert, Kerstin Lindblad-Toh, Jaime F. Modiano, Matthew Breen

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Recognition of the domestic dog as a model for the comparative study of human genetic traits has led to major advances in canine genomics. The pathophysiological similarities shared between many human and dog diseases extend to a range of cancers. Human tumors frequently display recurrent chromosome aberrations, many of which are hallmarks of particular tumor subtypes. Using a range of molecular cytogenetic techniques we have generated evidence indicating that this is also true of canine tumors. Detailed knowledge of these genomic abnormalities has the potential to aid diagnosis, prognosis, and the selection of appropriate therapy in both species. We recently improved the efficiency and resolution of canine cancer cytogenetics studies by developing a small-scale genomic microarray comprising a panel of canine BAC clones representing subgenomic regions of particular interest. We have now extended these studies to generate a comprehensive canine comparative genomic hybridization (CGH) array that comprises 1158 canine BAC clones ordered throughout the genome with an average interval of 2 Mb. Most of the clones (84.3%) have been assigned to a precise cytogenetic location by fluorescence in situ hybridization (FISH), and 98.5% are also directly anchored within the current canine genome assembly, permitting direct translation from cytogenetic aberration to DNA sequence. We are now using this resource routinely for high-throughput array CGH and single-locus probe analysis of a range of canine cancers. Here we provide examples of the varied applications of this resource to tumor cytogenetics, in combination with other molecular cytogenetic techniques.

Original languageEnglish (US)
Pages (from-to)1831-1837
Number of pages7
JournalGenome research
Volume15
Issue number12
DOIs
StatePublished - Dec 2005

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