Construction and direct electrochemistry of orientation controlled laccase electrode

Ying Li, Jiwei Zhang, Xirong Huang, Tianhong Wang

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29 Scopus citations


A laccase has multiple redox centres. Chemisorption of laccases on a gold electrode through a polypeptide tag introduced at the protein surface provides an isotropic orientation of laccases on the Au surface, which allows the orientation dependent study of the direct electrochemistry of laccase. In this paper, using genetic engineering technology, two forms of recombinant laccase which has Cys-6×His tag at the N or C terminus were generated. Via the Au-S linkage, the recombinant laccase was assembled orientationally on gold electrode. A direct electron transfer and a bioelectrocatalytic activity toward oxygen reduction were observed on the two orientation controlled laccase electrodes, but their electrochemical behaviors were found to be quite different. The orientation of laccase on the gold electrode affects both the electron transfer pathway and the electron transfer efficiency of O2 reduction. The present study is helpful not only to the in-depth understanding of the direct electrochemistry of laccase, but also to the development of laccase-based biofuel cells.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Mar 28 2014

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support from the State Key Laboratory of Microbial Technology of China, the National Natural Science Foundation of China ( 20973103 and 21173133 ), and the National Basic Research Program of China ( 2011CB707400 ).

Copyright 2014 Elsevier B.V., All rights reserved.


  • Direct electron transfer
  • Electron transfer pathway
  • Gold electrode
  • Oriented immobilization
  • Recombinant laccase


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