Constitution of rat glomerular mesangial cell stably expressing decorin gene

Hui Jun Wang; Zhi Gang Zhang; Guang Ping Chen; Wen Sheng Lin; Qi Chen; Mu Yi Guo

Constitution of rat glomerular mesangial cell stably expressing decorin gene

Hui Jun Wang, Zhi Gang Zhang, Guang Ping Chen, Wen Sheng Lin, Qi Chen, Mu Yi Guo

Neuroscience

Research output: Contribution to journal › Article › peer-review

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Abstract

Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.

Original language	English (US)
Pages (from-to)	97-99+102
Journal	Fudan University Journal of Medical Sciences
Volume	28
Issue number	2
State	Published - Dec 1 2001

Keywords

Decorin
Gene transfection
Mesangial cell

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title = "Constitution of rat glomerular mesangial cell stably expressing decorin gene",

abstract = "Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.",

keywords = "Decorin, Gene transfection, Mesangial cell",

author = "Wang, {Hui Jun} and Zhang, {Zhi Gang} and Chen, {Guang Ping} and Lin, {Wen Sheng} and Qi Chen and Guo, {Mu Yi}",

year = "2001",

month = dec,

day = "1",

language = "English (US)",

volume = "28",

pages = "97--99+102",

journal = "Fudan University Journal of Medical Sciences",

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publisher = "Fudan University",

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}

TY - JOUR

T1 - Constitution of rat glomerular mesangial cell stably expressing decorin gene

AU - Wang, Hui Jun

AU - Zhang, Zhi Gang

AU - Chen, Guang Ping

AU - Lin, Wen Sheng

AU - Chen, Qi

AU - Guo, Mu Yi

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.

AB - Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.

KW - Decorin

KW - Gene transfection

KW - Mesangial cell

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JO - Fudan University Journal of Medical Sciences

JF - Fudan University Journal of Medical Sciences

IS - 2

ER -

Constitution of rat glomerular mesangial cell stably expressing decorin gene

Abstract

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