Constitution of rat glomerular mesangial cell stably expressing decorin gene

Hui Jun Wang, Zhi Gang Zhang, Guang Ping Chen, Wen Sheng Lin, Qi Chen, Mu Yi Guo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.

Original languageEnglish (US)
Pages (from-to)97-99+102
JournalFudan University Journal of Medical Sciences
Volume28
Issue number2
StatePublished - Dec 1 2001

Keywords

  • Decorin
  • Gene transfection
  • Mesangial cell

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