Conserved T cell receptor V gene usage by uveitogenic T cells

Dale S Gregerson, Steven P. Fling, Carmen F. Merryman, Xiangming Zhang, Xiaobin Li, Ellen Heber-Katz

Research output: Contribution to journalArticle

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Abstract

Retinal S-antigen is widely used to study the LEW rat model of experimental autoimmune uveoretinitis (EAU). In this report, we have examined the T cell receptor V gene usage of several T cell lines recognizing either pathogenic or nonpathogenic sites on S-antigen to determine whether the Vα510 and Vβ510 rat homologues of the murine Vα2 and Vβ8 families, respectively, are used by uveitogenic T cells. Using cDNA probes for a LEW rat T cell receptor specific for the encephalitogenic determinant of myelin basic protein, we have found that in the retinal S-antigen/EAU model for autoimmune disease, pathogenicity correlates with usage of those rat V genes. Thus, all of the pathogenic lines were found to express T cell receptors of the Vβ510 and Vα510 families; conversely, Vβ510 usage was not detected in any of the nonpathogenic lines. Usage of these V regions has been associated with pathogenicity in the murine and rat models of experimental autoimmune encephalomyelitis, and now with S-antigen-induced EAU.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalClinical Immunology and Immunopathology
Volume58
Issue number1
DOIs
StatePublished - Jan 1 1991

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T-Cell Receptor Genes
T-Lymphocytes
Arrestin
T-Cell Antigen Receptor
Virulence
Antigens
Myelin Basic Protein
Autoimmune Experimental Encephalomyelitis
Autoimmune Diseases
Theoretical Models
Complementary DNA
Cell Line
Genes

Cite this

Conserved T cell receptor V gene usage by uveitogenic T cells. / Gregerson, Dale S; Fling, Steven P.; Merryman, Carmen F.; Zhang, Xiangming; Li, Xiaobin; Heber-Katz, Ellen.

In: Clinical Immunology and Immunopathology, Vol. 58, No. 1, 01.01.1991, p. 154-161.

Research output: Contribution to journalArticle

Gregerson, Dale S ; Fling, Steven P. ; Merryman, Carmen F. ; Zhang, Xiangming ; Li, Xiaobin ; Heber-Katz, Ellen. / Conserved T cell receptor V gene usage by uveitogenic T cells. In: Clinical Immunology and Immunopathology. 1991 ; Vol. 58, No. 1. pp. 154-161.
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