Conserved T cell receptor V gene usage by uveitogenic T cells

Dale S. Gregerson, Steven P. Fling, Carmen F. Merryman, Xiangming Zhang, Xiaobin Li, Ellen Heber-Katz

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Retinal S-antigen is widely used to study the LEW rat model of experimental autoimmune uveoretinitis (EAU). In this report, we have examined the T cell receptor V gene usage of several T cell lines recognizing either pathogenic or nonpathogenic sites on S-antigen to determine whether the Vα510 and Vβ510 rat homologues of the murine Vα2 and Vβ8 families, respectively, are used by uveitogenic T cells. Using cDNA probes for a LEW rat T cell receptor specific for the encephalitogenic determinant of myelin basic protein, we have found that in the retinal S-antigen/EAU model for autoimmune disease, pathogenicity correlates with usage of those rat V genes. Thus, all of the pathogenic lines were found to express T cell receptors of the Vβ510 and Vα510 families; conversely, Vβ510 usage was not detected in any of the nonpathogenic lines. Usage of these V regions has been associated with pathogenicity in the murine and rat models of experimental autoimmune encephalomyelitis, and now with S-antigen-induced EAU.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalClinical Immunology and Immunopathology
Volume58
Issue number1
DOIs
StatePublished - Jan 1991

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