Conserved MicroRNAs in Chinese hamster ovary cell lines

Kathryn C. Johnson, Nitya M. Jacob, Peter Morin Nissom, Matthias Hackl, Lim Hseuh Lee, Miranda Yap, Wei Shou Hu

Research output: Contribution to journalLetter

38 Scopus citations

Abstract

MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalBiotechnology and bioengineering
Volume108
Issue number2
DOIs
StatePublished - Feb 1 2011

Keywords

  • CHO cells
  • Chinese hamster
  • Illumina sequencing
  • MiRNA
  • MicroRNA

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    Johnson, K. C., Jacob, N. M., Nissom, P. M., Hackl, M., Lee, L. H., Yap, M., & Hu, W. S. (2011). Conserved MicroRNAs in Chinese hamster ovary cell lines. Biotechnology and bioengineering, 108(2), 475-480. https://doi.org/10.1002/bit.22940