Conserved MicroRNAs in Chinese hamster ovary cell lines

Kathryn C. Johnson; Nitya M. Jacob; Peter Morin Nissom; Matthias Hackl; Lim Hseuh Lee; Miranda Yap; Wei Shou Hu

doi:10.1002/bit.22940

Conserved MicroRNAs in Chinese hamster ovary cell lines

Kathryn C. Johnson, Nitya M. Jacob, Peter Morin Nissom, Matthias Hackl, Lim Hseuh Lee, Miranda Yap, Wei Shou Hu

Research output: Contribution to journal › Letter › peer-review

41 Scopus citations

Abstract

MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.

Original language	English (US)
Pages (from-to)	475-480
Number of pages	6
Journal	Biotechnology and bioengineering
Volume	108
Issue number	2
DOIs	https://doi.org/10.1002/bit.22940
State	Published - Feb 2011

Keywords

CHO cells
Chinese hamster
Illumina sequencing
MiRNA
MicroRNA

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10.1002/bit.22940

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abstract = "MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.",

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AU - Johnson, Kathryn C.

AU - Jacob, Nitya M.

AU - Nissom, Peter Morin

AU - Hackl, Matthias

AU - Lee, Lim Hseuh

AU - Yap, Miranda

AU - Hu, Wei Shou

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N2 - MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.

AB - MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.

KW - CHO cells

KW - Chinese hamster

KW - Illumina sequencing

KW - MiRNA

KW - MicroRNA

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Conserved MicroRNAs in Chinese hamster ovary cell lines

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