Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

Kenneth W. Adolph

doi:10.1016/0014-5793(84)80171-4

Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

Kenneth W. Adolph

Molecular Biology (TMED)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with ¹²⁵I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with ¹²⁵I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.

Original language	English (US)
Pages (from-to)	211-215
Number of pages	5
Journal	FEBS Letters
Volume	165
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(84)80171-4
State	Published - Jan 9 1984

Bibliographical note

Funding Information:
This researchw ass upportedb y a grant from the National Instituteso f Health and by funds from the Biomedical Research Support Grant of the University of MinnesotaM edical School.

Keywords

Antiserum
Chromatin
Electrophoresis
HeLa cell
Metaphase chromosome
Nonhistone

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10.1016/0014-5793(84)80171-4

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title = "Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes",

abstract = "The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90\% for chromatin.",

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T1 - Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

AU - Adolph, Kenneth W.

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N2 - The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.

AB - The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.

KW - Antiserum

KW - Chromatin

KW - Electrophoresis

KW - HeLa cell

KW - Metaphase chromosome

KW - Nonhistone

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DO - 10.1016/0014-5793(84)80171-4

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AN - SCOPUS:0021758416

SN - 0014-5793

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EP - 215

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

Abstract

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