Abstract
The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.
Original language | English (US) |
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Pages (from-to) | 211-215 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 165 |
Issue number | 2 |
DOIs | |
State | Published - Jan 9 1984 |
Bibliographical note
Funding Information:This researchw ass upportedb y a grant from the National Instituteso f Health and by funds from the Biomedical Research Support Grant of the University of MinnesotaM edical School.
Keywords
- Antiserum
- Chromatin
- Electrophoresis
- HeLa cell
- Metaphase chromosome
- Nonhistone