The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Jan 9 1984|
Bibliographical noteFunding Information:
This researchw ass upportedb y a grant from the National Instituteso f Health and by funds from the Biomedical Research Support Grant of the University of MinnesotaM edical School.
- HeLa cell
- Metaphase chromosome