Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy

John J. Lemasters, Ting Qian, Steven P. Elmore, Lawrence C. Trost, Yoshiya Nishimura, Brian Herman, Cynthia A. Bradham, David A. Brenner, Anna Liisa Nieminen

Research output: Contribution to journalShort survey

63 Citations (Scopus)

Abstract

Onset of the cyclosporin-A-sensitive mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by laser scanning confocal microscopy. The MPT is a causative event in many types of necrotic and apoptotic cell death, including oxidative stress, ischemia/reperfusion injury, Ca 2+ ionophore toxicity and tumor necrosis factor alpha (TNFα) induced apoptosis, and may contribute to Reye's-related drag toxicity. Pyridine nucleotide oxidation, mitochondrial generation of reactive oxygen species, and increased mitochondrial Ca 2+ and pH can each promote onset of the MPT in situ. The MPT can also be directly visualized during TNFα-induced apoptosis to hepatocytes. Mitochondria spontaneously depolarize in situ after nutrient deprivation before entering an acidic lysosomal compartment, suggesting that the MPT precedes the normal process of mitochondrial autophagy. We propose a model in which onset of the MPT to increasing numbers of mitochondria leads progressively to autophagy, apoptosis and necrotic cell death.

Original languageEnglish (US)
Pages (from-to)283-285
Number of pages3
JournalBioFactors
Volume8
Issue number3-4
DOIs
StatePublished - Jan 1 1998

Fingerprint

Mitochondria
Confocal microscopy
Autophagy
Confocal Microscopy
Permeability
Cells
Cell death
Apoptosis
Toxicity
Tumor Necrosis Factor-alpha
Oxidative stress
Ionophores
Cyclosporine
Nutrients
Drag
Reactive Oxygen Species
Cell Death
Nucleotides
Scanning
Oxidation

Cite this

Lemasters, J. J., Qian, T., Elmore, S. P., Trost, L. C., Nishimura, Y., Herman, B., ... Nieminen, A. L. (1998). Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy. BioFactors, 8(3-4), 283-285. https://doi.org/10.1002/biof.5520080316

Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy. / Lemasters, John J.; Qian, Ting; Elmore, Steven P.; Trost, Lawrence C.; Nishimura, Yoshiya; Herman, Brian; Bradham, Cynthia A.; Brenner, David A.; Nieminen, Anna Liisa.

In: BioFactors, Vol. 8, No. 3-4, 01.01.1998, p. 283-285.

Research output: Contribution to journalShort survey

Lemasters, JJ, Qian, T, Elmore, SP, Trost, LC, Nishimura, Y, Herman, B, Bradham, CA, Brenner, DA & Nieminen, AL 1998, 'Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy', BioFactors, vol. 8, no. 3-4, pp. 283-285. https://doi.org/10.1002/biof.5520080316
Lemasters, John J. ; Qian, Ting ; Elmore, Steven P. ; Trost, Lawrence C. ; Nishimura, Yoshiya ; Herman, Brian ; Bradham, Cynthia A. ; Brenner, David A. ; Nieminen, Anna Liisa. / Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy. In: BioFactors. 1998 ; Vol. 8, No. 3-4. pp. 283-285.
@article{b5de2b95a9454114ab2d432b9a0c67a8,
title = "Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy",
abstract = "Onset of the cyclosporin-A-sensitive mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by laser scanning confocal microscopy. The MPT is a causative event in many types of necrotic and apoptotic cell death, including oxidative stress, ischemia/reperfusion injury, Ca 2+ ionophore toxicity and tumor necrosis factor alpha (TNFα) induced apoptosis, and may contribute to Reye's-related drag toxicity. Pyridine nucleotide oxidation, mitochondrial generation of reactive oxygen species, and increased mitochondrial Ca 2+ and pH can each promote onset of the MPT in situ. The MPT can also be directly visualized during TNFα-induced apoptosis to hepatocytes. Mitochondria spontaneously depolarize in situ after nutrient deprivation before entering an acidic lysosomal compartment, suggesting that the MPT precedes the normal process of mitochondrial autophagy. We propose a model in which onset of the MPT to increasing numbers of mitochondria leads progressively to autophagy, apoptosis and necrotic cell death.",
author = "Lemasters, {John J.} and Ting Qian and Elmore, {Steven P.} and Trost, {Lawrence C.} and Yoshiya Nishimura and Brian Herman and Bradham, {Cynthia A.} and Brenner, {David A.} and Nieminen, {Anna Liisa}",
year = "1998",
month = "1",
day = "1",
doi = "10.1002/biof.5520080316",
language = "English (US)",
volume = "8",
pages = "283--285",
journal = "BioFactors",
issn = "0951-6433",
publisher = "Wiley-Blackwell",
number = "3-4",

}

TY - JOUR

T1 - Confocal microscopy of the mitochondrial permeability transition in necrotic cell killing, apoptosis and autophagy

AU - Lemasters, John J.

AU - Qian, Ting

AU - Elmore, Steven P.

AU - Trost, Lawrence C.

AU - Nishimura, Yoshiya

AU - Herman, Brian

AU - Bradham, Cynthia A.

AU - Brenner, David A.

AU - Nieminen, Anna Liisa

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Onset of the cyclosporin-A-sensitive mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by laser scanning confocal microscopy. The MPT is a causative event in many types of necrotic and apoptotic cell death, including oxidative stress, ischemia/reperfusion injury, Ca 2+ ionophore toxicity and tumor necrosis factor alpha (TNFα) induced apoptosis, and may contribute to Reye's-related drag toxicity. Pyridine nucleotide oxidation, mitochondrial generation of reactive oxygen species, and increased mitochondrial Ca 2+ and pH can each promote onset of the MPT in situ. The MPT can also be directly visualized during TNFα-induced apoptosis to hepatocytes. Mitochondria spontaneously depolarize in situ after nutrient deprivation before entering an acidic lysosomal compartment, suggesting that the MPT precedes the normal process of mitochondrial autophagy. We propose a model in which onset of the MPT to increasing numbers of mitochondria leads progressively to autophagy, apoptosis and necrotic cell death.

AB - Onset of the cyclosporin-A-sensitive mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by laser scanning confocal microscopy. The MPT is a causative event in many types of necrotic and apoptotic cell death, including oxidative stress, ischemia/reperfusion injury, Ca 2+ ionophore toxicity and tumor necrosis factor alpha (TNFα) induced apoptosis, and may contribute to Reye's-related drag toxicity. Pyridine nucleotide oxidation, mitochondrial generation of reactive oxygen species, and increased mitochondrial Ca 2+ and pH can each promote onset of the MPT in situ. The MPT can also be directly visualized during TNFα-induced apoptosis to hepatocytes. Mitochondria spontaneously depolarize in situ after nutrient deprivation before entering an acidic lysosomal compartment, suggesting that the MPT precedes the normal process of mitochondrial autophagy. We propose a model in which onset of the MPT to increasing numbers of mitochondria leads progressively to autophagy, apoptosis and necrotic cell death.

UR - http://www.scopus.com/inward/record.url?scp=0032406623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032406623&partnerID=8YFLogxK

U2 - 10.1002/biof.5520080316

DO - 10.1002/biof.5520080316

M3 - Short survey

VL - 8

SP - 283

EP - 285

JO - BioFactors

JF - BioFactors

SN - 0951-6433

IS - 3-4

ER -